As part of the Hillsborough Health Equity Plan, a formative research initiative was conducted to increase the uptake of pre-exposure prophylaxis (PrEP) among Black and Latinx men who have sex with men (MSM) aged 25–34 in Hillsborough County, Florida. The study explored patient and provider experiences within on-demand care settings, such as urgent care clinics and emergency departments (EDs), where opportunities for PrEP intervention are often missed.